Title:
【発明の名称】腫瘍壊死因子の精製、製造および使用法
Document Type and Number:
Japanese Patent JP2557053
Kind Code:
B2
Abstract:
A process for purifying mammalian TNF from a composition comprising the steps of (a) precipitating crude TNF from the composition; (b) fractionating TNF by anion exchange chromatography at a mild alkaline pH; (c) fractioning TNF by gel filtration; and (d) fractionating TNF by anion exchange chromatography at a slightly acidic pH.
Inventors:
FUAIAAZU UORUTA SHII
FURANSEN RUSHIA EMU
TABERUNIA JAN HONOORU REO
MARUMENAUTO ANII RUISU MARII
BAN DERU HEIDEN JOZE
ARETSUTO BERUNAARU
KAWASHIMA ERITSUKU EICHI
FURANSEN RUSHIA EMU
TABERUNIA JAN HONOORU REO
MARUMENAUTO ANII RUISU MARII
BAN DERU HEIDEN JOZE
ARETSUTO BERUNAARU
KAWASHIMA ERITSUKU EICHI
Application Number:
JP50055086A
Publication Date:
November 27, 1996
Filing Date:
December 19, 1985
Export Citation:
Assignee:
BAIOJEN INC
International Classes:
A61K38/00; A61K38/19; A61K38/21; A61K45/00; A61P33/02; G01N33/577; A61P35/00; C07H21/00; C07H21/04; C07K1/18; C07K14/00; C07K14/52; C07K14/525; C07K14/555; C07K14/56; C07K14/565; C07K14/57; C12N15/00; C12N15/09; C12N15/28; C12N15/70; C12P21/00; C12P21/02; C12Q1/02; C12R1/19; A61K; C07H; C07K; C12N; C12P; (IPC1-7): C12P21/02
Domestic Patent References:
| JP60185799A |
Other References:
Ｇｅｎｅ，１８，２９７−３０７（１９８２）
Ｎａｔｕｒｅ，３１２，７２１−７２４（１９８４）
Ｎａｔｕｒｅ，３１３，８０３−８０６（１９８５）
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，７９，４９３７−４９４１（１９８２）
Ｓｃｉｅｎｃｅ，２２８，１４９−１５４（１９８５）
Ｎａｔｕｒｅ，３１２，７２１−７２４（１９８４）
Ｎａｔｕｒｅ，３１３，８０３−８０６（１９８５）
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，７９，４９３７−４９４１（１９８２）
Ｓｃｉｅｎｃｅ，２２８，１４９−１５４（１９８５）
Attorney, Agent or Firm:
Haruo Hamada