Title:
DETECTION OF IMMOBILIZED NUCLEIC ACID
Document Type and Number:
Japanese Patent JP2011099862
Kind Code:
A
Abstract:
To provide a method for determining the presence of immobilized nucleic acid, by employing: unsymmetrical cyanine dyes that are derivatives of thiazole orange; a staining solution; and carefully-selected fluorogenic compounds that are characterized as being essentially non-genotoxic.
The method includes processes of: immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi-solid support; contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound; and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined.
Inventors:
BEAUDET MATTHEW P
COX GREGORY W
YUE STEPHEN
COX GREGORY W
YUE STEPHEN
Application Number:
JP2010255930A
Publication Date:
May 19, 2011
Filing Date:
November 16, 2010
Export Citation:
Assignee:
MOLECULAR PROBES INC
International Classes:
G01N33/50; C07D413/02; C07D417/02; C07D417/06; C09B23/00; C09B23/02; C09B67/44; C12Q1/68; G01N33/48; G01N33/533; G01N21/78
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi
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