PURPOSE: To detect the protein separated in a gel at a high speed and with high sensitivity and good reproducibility by using N, N' diethylamine and 2, 4 dichlornaphthol as a precursor of a dye.
CONSTITUTION: The gel in which the protein is separated by a two-dimensional electrophoresis method is installed in a dyeing cell (e) and a valve V1 is opened to inject a hemin soln. into the cell (e) in order to adsorb the hemin to the protein in the gel. The hemin is thus adsorbed onto the protein. A valve V5 is then opened to transfer the hemin soln. to a waste liquid storage tank (g) and a valve V2 is opened to inject a phosphoric acid soln. into the cell (e) to clean the excess hemin in the background part where the protein does not exist with the phosphoric acid soln. A valve V5 is opened, after the cleaning, to transfer the phosphoric acid into the tank (g). A valve V3 is then opened to inject the mixture composed of the N, N' diethylamine and 2, 4 dichlornaphthol which is the precursor of the dye for dyeing the protein into the cell (e). Hydrogen peroxide soln. which is an initiator for the oxidation coupling reactino of the two precursors is injected into the cell by opening a valve V4 to cause the dyeing reaction, by which the protein is detected.
MURAKAMI SEI
SONODA HIROSHI
TOO KUNIHIKO
HITACHI TECHNO ENG