Title:
DETERMINATION OF BOD AND APPARATUS THEREFOR
Document Type and Number:
Japanese Patent JP3097732
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To readily, quickly and correctly determine BOD of industrial waste water and other waste water to be treated by determining a luminous amount by assimilation of an organic substance of a fungus having a luminous gene group containing a luciferase gene and a substrate synthesized gene.
SOLUTION: A microorganism suspension wherein a fungus (e.g.; Escherichia coli) to which a luminous gene group containing a luciferase gene such as lux A or lux B and a substrate synthesized gene such as lux C-lux E of a luminous substrate such as luciferin are introduced is suspended in a buffer solution is set in a lumi-counter by filling in a glass microtube, a sample of various waster waters is added to the suspension, a luminous amount of the fungus by assimilation of an organic substance is determined by a digital voltmeter as a number of electrons emitted by a photoelectric effect of a photomulticounter in the lumi-counter, the resultant data are transmitted to a personal computer and a data calculation is operated to readily, quickly and correctly determine BOD of industrial waste water and other waste water to be treated.
Inventors:
Eiichi Tamiya
Tomotsugu Ando
Yuji Murakami
Kenji Yokoyama
Toyama Masamitsu
Tomotsugu Ando
Yuji Murakami
Kenji Yokoyama
Toyama Masamitsu
Application Number:
JP23461595A
Publication Date:
October 10, 2000
Filing Date:
August 22, 1995
Export Citation:
Assignee:
Ishikawa Manufacturing Co., Ltd.
Eiichi Tamiya
Eiichi Tamiya
International Classes:
G01N21/76; C12M1/34; C12N1/21; C12Q1/02; C12Q1/66; G01N33/18; C12R1/19; (IPC1-7): C12Q1/02; C12M1/34; C12Q1/66; G01N21/76; G01N33/18
Attorney, Agent or Firm:
Kazuo Kumada