Determination of in-solution binding kinetics based on immunoassay

Japanese Patent JP6336980

B2

Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.

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WO/1993/013127	PEPTIDES IMMUNOCHEMICALLY REACTIVE WITH ANTIBODIES DIRECTED AGAINST HEPATITIS NON-A, NON-B VIRUS
JP2000221191	DIAGNOSTIC MARKER FOR HUMAN DISORDERS

Dar Uwe
Jordan Gregor
Stark roland

JP2015525837A

June 06, 2018

August 02, 2013

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F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT

G01N33/53; A61K39/395

WO2011038301A2

AZIMZADEH, A.,MEASUREMENT OF AFFINITY OF VIRAL MONOCLONAL ANTIBODIES BY ELISA TITRATION OF FREE ANTIBODY IN EQUILIBRIUM MIXTURES,JOURNAL OF IMMUNOLOGICAL METHODS,ELSEVIER SCIENCE PUBLISHERS B.V.,1991年 8月 9日,V141 N2,P199-208
STAACK, ROLAND F.,MATHEMATICAL SIMULATIONS FOR BIOANALYTICAL ASSAY DEVELOPMENT: THE (UN-)NECESSITY AND (IM-)POSSIBILITY OF FREE DRUG QUANTIFICATION,BIOANALYSIS,FUTURE SCIENCE,2012年 2月 1日,V4 N4,P381-395

Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto

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