Title:
ジホスホメバロン酸デカルボキシラーゼ変異体、及びそれを用いたオレフィン化合物の製造方法
Document Type and Number:
Japanese Patent JP7054092
Kind Code:
B2
Abstract:
The present invention aims to provide a method which enables olefin compound production with a high productivity, and an enzyme used in the method. The present inventors introduced mutations involving amino acid substitution into various sites of diphosphomevalonate decarboxylase (MVD) to prepare multiple MVD variants. Then, the present inventors evaluated these variants in terms of catalytic activity for producing an olefin compound such as isoprene, and have found as a result that the catalytic activity is improved when serine at position 153 and threonine at position 209 are each substituted with a different amino acid. In addition, the present inventors have revealed that the catalytic activity of the variants is further improved when glycine at position 152 is further substituted with a different amino acid.
Inventors:
Ryoko Orishita
Satoshi Shirai
Kazuhiro Takahashi
Rinza Misao
Yusuke Tanabe
Satoshi Shirai
Kazuhiro Takahashi
Rinza Misao
Yusuke Tanabe
Application Number:
JP2018565547A
Publication Date:
April 13, 2022
Filing Date:
January 30, 2018
Export Citation:
Assignee:
RIKEN
Zeon Corporation
THE YOKOHAMA RUBBER CO.,LTD.
Zeon Corporation
THE YOKOHAMA RUBBER CO.,LTD.
International Classes:
C12N15/60; C07K14/395; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12N15/63; C12P5/02; C12P21/02
Domestic Patent References:
| JP2015501660A |
Foreign References:
| WO2015021045A2 | ||||
| WO2015004211A2 |
Other References:
Appl. Environ.Microbiol., 2010, Vol.76, No.24, pp.8004-8010
日本農芸化学会2016年度大会講演要旨集, 2016.03.05, 発表番号:2J003
日本農芸化学会2016年度大会講演要旨集, 2016.03.05, 発表番号:2J003
Attorney, Agent or Firm:
Patent Business Corporation St. Crest International Patent Office