Title:
シラン処理シリカ基質を用いた溶解物クリアランスおよび核酸単離
Document Type and Number:
Japanese Patent JP4198461
Kind Code:
B2
Abstract:
A method is provided for using a silanized silica matrix to isolate a target nucleic acids, such as plasmid DNA, fragments of DNA, chrmosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or non-target nucleic acids. The silanized silica matrix comprises a silica based solid phase and a plurality of silane ligands covalently attached to the surface of the solid phase. Non-target material absorbs to the silanized silica matrix in the presence of a sufficient concentration of chaotropic salt, while target nucleic acids adsorb to the matrix under other solution conditions. The method of using the sinalized silica matrix of the present invention can be used to clear solutions of disrupted biological material, and to isolate nucleic acids therefrom or from other solutions containing nucleic acids and at least one contaminant.
Inventors:
Bitner Rex M
Simpson daniel jay
Fleming Rhoderick G
Coller Susan Sea
Simpson daniel jay
Fleming Rhoderick G
Coller Susan Sea
Application Number:
JP2002542074A
Publication Date:
December 17, 2008
Filing Date:
November 08, 2001
Export Citation:
Assignee:
Promega Corporation
International Classes:
C12N15/09; G01N33/48; B01D15/08; B01D43/00; B01J20/10; B03C1/00; C07B61/00; C07H21/00; C12N15/10
Domestic Patent References:
| JP63150294A | ||||
| JP57003043A | ||||
| JP7501222A | ||||
| JP5502940A | ||||
| JP2002543979A | ||||
| JP2002543835A | ||||
| JP2002502606A | ||||
| JP2002512252A |
Foreign References:
| WO1993010162A1 |
Other References:
Proc.SPIE,2000年 3月,Vol.3926,p.126-133
URL: http://www.promega.co.jp/jp/jp_tech/jp_manuals/wsv96.pdf,1999年11月,URL,URL: http://www.promega.co.jp/jp/jp_tech/jp_manuals/wsv96.pdf
URL: http://www.promega.co.jp/jp/jp_tech/jp_manuals/wsv96.pdf,1999年11月,URL,URL: http://www.promega.co.jp/jp/jp_tech/jp_manuals/wsv96.pdf
Attorney, Agent or Firm:
Minoru Nakamura
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda