Title:
DNA ENCODING HUMAN GROWTH HORMONE FUNCTIONALLY BONDED TO DNA ENCODING SIGNAL SEQUENCE
Document Type and Number:
Japanese Patent JP2521413
Kind Code:
B2
Abstract:
PURPOSE: To obtain a DNA a mature human growth hormone, in relation to a method for producing a protein in a bacterium, in detail, operabily ligated to a DNA encoding a signal sequence of alkaliphosphatase or enterotoxin.
CONSTITUTION: A eukaryotic protein is expressed by the following method to secrete a eukaryotic periplasmic space. (a) A vector for expressing a direct hybrid to be secreted, containing a sequence in which 3' terminal of DNA encoding a eukaryotic secretion signal sequence and is bonded to 5' terminal of a DNA encoding a mature protein, is constructed, (b) a eukaryotic host molding organism is transformed with the vector of the process (a), (c) the host transformed by the process (b) is cultured and (d) a mature protein is accumulated in periplasm of the host.
Inventors:
BARII RONARUDO BOSHUNAA
CHUNNNAN CHAN
GUREGORII ROORENSU GUREI
HERUBERUTO RUISU HAINEKAA
NANSHII SHADOITSUKU MAKUFUAARANDO
KENESU CHAARUZU ORUSON
RONNCHAN PEI
MAIKERU UIRAADO REI
CHUNNNAN CHAN
GUREGORII ROORENSU GUREI
HERUBERUTO RUISU HAINEKAA
NANSHII SHADOITSUKU MAKUFUAARANDO
KENESU CHAARUZU ORUSON
RONNCHAN PEI
MAIKERU UIRAADO REI
Application Number:
JP7316994A
Publication Date:
August 07, 1996
Filing Date:
April 12, 1994
Export Citation:
Assignee:
GENENTECH INC
International Classes:
C07K14/24; C07K14/61; C12N1/00; C12N5/00; C12N15/00; C12N15/09; C12N15/62; C12N1/21; C12N15/70; C12P21/02; C12R1/19; (IPC1-7): C12N15/09
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)