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Title:
DNA SEQUENCE
Document Type and Number:
Japanese Patent JP3193917
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To provide a DNA sequence containing a structural gene encoding xylitol dehydrogenase (XYL2) and capable of expressrng the polypeptide in a microorganism, a combination of the DNA sequence, a vector, the microorganism, a manufacturing method for the XYL2 and the XYL2 as a protein and a producing method for NADP+ from NADPH by using a xylose resource, which is conventionally considered as a waste, as a carbon resource for ethanol fermentation or biomass production.
SOLUTION: This invention discloses cloning of DNA encoding the XYL2 which is a key for utilization of xylose, providing a tool useful for gene expression such as promoter or the like, a vector for transformation of a host cell mainly comprising a yeast, the host cell, an enzyme obtained by the expression and condition setting to succeed anaerobic fermentation. Microorganisms preferably used are Saccharomyces cerevisiae or Schizosaccharomyces probe.


Inventors:
Alexander W. M. Strasser
Cornelis P. Holenberg
Mickle Von Sericity-Van Trap
Peter cotter
Rene Amore
Michael pion tech
Yutta Hagedorn
Application Number:
JP2000276227A
Publication Date:
July 30, 2001
Filing Date:
March 26, 1991
Export Citation:
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Assignee:
Line Biotech Gesellschaft Fuhl Neue Biotechnologie Prozesse Und Prodocte Mbha
International Classes:
C07K14/00; C12N1/14; C12N1/19; C12N1/21; C12N9/02; C12N9/04; C12N15/53; C12N15/81; C12P7/06; C12P7/10; C12N15/09; C12P21/02; C12R1/01; C12R1/645; C12R1/84; C12R1/865; (IPC1-7): C12N15/09; C12N1/19; C12N9/04; C12P7/06
Domestic Patent References:
JP60199383A
JP6163291A
Other References:
【文献】Abstr.Annu.Meet.Am.Soc.Microbiol.,Vol.86(1986)p.262
Attorney, Agent or Firm:
Akira Asamura (2 outside)