DOCOSAHEXAENOIC ACID AND DOCOSAHEXAENOIC ACID-CONTAINING COMPOUND

Japanese Patent JP2006191931

A

To provide a method for producing dinoflagellate biomass containing single-cell oil for simple fare.

This method for producing the dinoflagellate biomass comprises (a) adding C.cohnii of about 106 cell/ml (0.5-1.0 g dry weight/L) to a fermentor containing in the initial stage nutrient solutions containing about artificial seawater of quarter or half salinity, 1-8% glucose, and 0.4-0.8% yeast extract, (b) culturing C.cohnii at 15°C-34°C at 5.0-9.0 pH, (c) gradually adding glucose and yeast extract to the nutrient solutions for about 56 h, (d) further adding glucose to the nutrient solutions for about 16 h so as to induce C.cohnii to produce oil, (e) stirring and ventilating the fermentor so as to keep dissolved oxygen content to at least about 20% of aeration level through culturing, and (f) collecting C.cohnii after about 60 h to 90 h.

KAIRU DEIBITSUDO JIYON
RIIBU SUU EREN
SHIKOTSUTO BARERII JIYAKURIINU

JP2006017013A

July 27, 2006

January 26, 2006

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MARTEK BIOSCIENCES CORP

A23D9/007; A23K1/18; A23C9/152; A23D9/00; A23D9/02; A23K1/00; A23K1/16; A23K20/195; A23L1/30; A23L29/269; A61K9/48; A61K31/20; A61K31/202; A61K31/23; A61K31/232; A61K35/60; A61K36/02; A61K47/14; A61K47/44; A61P3/00; A61P3/02; A61P9/00; A61P9/10; C11B1/10; C11C3/00; C12N1/12; C12N1/13; C12N15/09; C12P1/00; C12P7/64; C12R1/89; C12R1/90

Hatsushi Shimizu
Koichi Niimi

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