To obtain a new water-soluble fullerene derivative having water solubility and capable of imparting electroconductivity to a double-stranded DNA fragment.
This water-soluble fullerene derivative is represented by formula I (R1 and R3 are each a 2-10C heterocyclic group or the like containing 1-4 heteroatoms selected from N, S and P; R2 is H, a 2-7C acyl or the like; X is a counteranion of a cationic group), e.g. a compound represented by formula II. The derivative represented by formula I can be obtained in good yield by carrying out an alkylation, etc., of a precursor of the water-soluble fullerene derivative, e.g. N-methyl-2-(4'-N-pyridyl)-3,4-fulleropyridine with an alkyl triflate, etc., in a solvent such as chlorobenzene or fluorobenzene. The water-soluble fullerene derivative represented by formula I can be brought into contact with a double-stranded DNA fragment to thereby afford the double-stranded DNA fragment having electroconductivity. The resultant double-stranded DNA fragment can be utilized for quantitative determination of a substrate such as glutathione or a cytochrome.
YAMASHITA KENICHI
TAKAGI MAKOTO
HATTA TAIZO
TSUGE OTOHIKO
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