Title:
液滴ベースの分析システム
Document Type and Number:
Japanese Patent JP2012503773
Kind Code:
A
Abstract:
A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
Inventors:
Billy Wayne Colston Jr.
Benjamin Joseph Hindson
Kevin Dean Ness
Donald Arthur Maskelier
Fred Paul Milanovich
Douglas N Modrin
Vincent Riot
Samuel Bird
Anthony Joseph McAlevich
Phillip BELGRADER
Benjamin Joseph Hindson
Kevin Dean Ness
Donald Arthur Maskelier
Fred Paul Milanovich
Douglas N Modrin
Vincent Riot
Samuel Bird
Anthony Joseph McAlevich
Phillip BELGRADER
Application Number:
JP2011529019A
Publication Date:
February 09, 2012
Filing Date:
September 23, 2009
Export Citation:
Assignee:
Quanta Life Incorporated
International Classes:
G01N35/08; C12M1/00; G01N21/47; G01N37/00; C12Q1/68; G01N21/64
Domestic Patent References:
| JP2007175002A | 2007-07-12 | |||
| JPH0835971A | 1996-02-06 | |||
| JP2010506136A | 2010-02-25 | |||
| JP2009538123A | 2009-11-05 | |||
| JP2009536313A | 2009-10-08 | |||
| JP2005130851A | 2005-05-26 |
Foreign References:
| US20080038810A1 | 2008-02-14 | |||
| US20030006140A1 | 2003-01-09 | |||
| WO2007121489A2 | 2007-10-25 | |||
| WO2007081385A2 | 2007-07-19 | |||
| WO2008063227A2 | 2008-05-29 |
Attorney, Agent or Firm:
Yasuhiko Murayama
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro