Title:
ダイナミックコーティング
Document Type and Number:
Japanese Patent JP4681177
Kind Code:
B2
Abstract:
The instant invention describes uncharged water-soluble polymers to suppress electroosmotic flow in capillary electrophoresis. The polymers in the instant invention are copolymers of various derivatives of acrylamide and methacrylamide monomers with various glycidyl group containing monomers e.g., dimethylacrylamide and allyl glycidyl ether-epoxy poly(DMA)-, copolymers of various derivatives of acrylamide and methacrylamide with various allyl group containing carbohydrates and various glycidyl group containing monomers, such as allyl beta-D-pyranoside (typically beta-D-glucopyranoside) or allyl beta-D-furanoside, allyl glycidyl ether-epoxy poly(AG-AA) and copolymers of four different monomers including various acryl and methacrylamide, various allyl group containing carbohydrates, various glycidyl group containing monomers and various diol group containing monomers, such as acrylamide, allyl beta-D-pyranoside (typically beta-D-galactopyranoside or N-allylgluconamide) or allyl beta-D-furanoside, allyl glycydyl ether and allyoxy-1,2 propanediol-epoxy poly(AGal-AA-APD). The subject polymers adsorb onto the capillary surface, forming a highly hydrophilic, dynamic coating that suppresses electroosmotic flow. The subject polymers may be used as a coating prior to use of an electrophoresis microchannel or may be included in a separation media contained within the capillary column. The instant coatings and media are especially suitable for applications pertaining to the electrophoretic separation of various biomolecules, such as protein and DNA.
Inventors:
Chiari, Marcella
Application Number:
JP2001518765A
Publication Date:
May 11, 2011
Filing Date:
August 21, 2000
Export Citation:
Assignee:
Chiari, Marcella
International Classes:
C09D133/24; B01D57/02; B03C5/00; C08F216/14; C08F220/54; C08F220/56; C09D129/00; C12N9/12; C12Q1/68; C12Q1/6869; G01N27/447
Domestic Patent References:
| JP2001500971A |
Foreign References:
| US5935401 |
Attorney, Agent or Firm:
Mitsuo Tanaka
Takuji Yamada
Mitsutaka Iwasaki
Takuji Yamada
Mitsutaka Iwasaki