Title:
電気泳動法
Document Type and Number:
Japanese Patent JP4503433
Kind Code:
B2
Abstract:
The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilized medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium; and to observe the results by analysis of one or more sections of the separation medium. In the present method, a disulphide-comprising compound is added before or during the procedure to make an excess of reactive disulphide groups accessible to react with the cysteine groups of the proteins and/or peptides all through the separation procedure. The present invention also relates to electrophoretic separation media that comprises reactive disulphide groups, such as polyacrylamide gels, and the use of a solution that comprises reactive disulphide groups to pretreat an electrophoretic separation medium.
Inventors:
Bierquist, Bengt
Olson, Ingmar
Ronnie Palmgren
Olson, Ingmar
Ronnie Palmgren
Application Number:
JP2004508936A
Publication Date:
July 14, 2010
Filing Date:
May 07, 2003
Export Citation:
Assignee:
GE Healthcare Bioscience Activolag
International Classes:
G01N27/447; B01D57/02; B03C5/00; C07K1/26
Domestic Patent References:
| JP2001505306A | ||||
| JP11502615A | ||||
| JP9512907A |
Foreign References:
| US6203680 |
Other References:
Feng Xu(外5名),Controlled Stepwise Reduction of Disulfide Bonds and Heat-Induced Modification of Wheat Dough Proteins,CEREAL CHEMISTRY,1999年12月12日,Vol. 76, No. 6,p. 931-937
Attorney, Agent or Firm:
Arakawa Satoshi
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa