To provide an endoscope system for allowing living tissues to be observed in real time without causing mismatch between excitation light source wavelength and light receiving fluorescent wavelength even when fluorescent observation is performed by arbitrarily combining many kinds of fluorescent substances, and thus to extend an application range of diagnosis using the endoscope system.

In acquiring a two-dimensional image by radiating a scanning light to a predetermined observation region of a subject, a plurality of light sources 19A, 19B, and 19C successively emit pulse light within a unit main scanning period corresponding to a unit pixel of the two-dimensional image, detection of returning light to the respective light sources 19A, 19B, and 19C is repeated in the main scanning direction and in the sub-scanning direction in synchronization with timing of pulse light emission by detectors 43A, 43B, and 43C within the unit main scanning period, and a plurality of two-dimensional images corresponding to the respective light sources 19A, 19B, and 19C are generated by scanning of one screen.