Title:
【発明の名称】エネルギー転移色素
Document Type and Number:
Japanese Patent JP2002523783
Kind Code:
A
Abstract:
Energy transfer dyes, their preparation, and their use as labels in biological systems is disclosed. The dyes are preferably in the form of cassettes which enable their attachment to a variety of biological materials. The dyes and the reagents that can be made from them offer a wide variety of fluorescent labels with large Stokes' shifts enabling their use in a variety of fluorescence applications over a wide range of the visible spectrum. The energy transfer dyes may be of the formula:
D1 - L1 -A-L2 - D2
wherein
D1 is a first dye moiety suitable as an acceptor or donor in an energy transfer arrangement; D2 is a second dye moiety suitable as a donor or acceptor in an energy transfer arrangement with the first dye; ```A comprises 5-20 linked atoms, wherein the linked atoms contain at least one aryl or heteroaryl group and wherein the atoms are independently selected from the group consisting of carbon, nitrogen, sulfur, oxygen, phosphorus;
```L1 and L2 comprise a group of linked atoms and L2 is adapted for attaching to a biological target. The following are specifically claimed 5-FAM-Phe-5-REG-11-ddUTP, 5-FAM-Phe-5-REG-11-ddCTP, 5-FAM-Phe-5-REG-11-ddATP, 5-FAM-Phe-5-REG-11-ddGTP, 5-FAM-Phe-5-ROX-11-ddCTP, 5-FAM-Phe-5-ROX-11-ddUTP, 5-FAM-Phe-5-ROX-11-ddATP, 5-FAM-Phe-5-ROX-11-ddGTP, 5-FAM-Phe-5-TAMARA-11-ddGTP, 5-FAM-Phe-5-TAMARA-11-ddATP, 5-FAM-Phe-5-TAMARA-11-ddCTP, 5-FAM-Phe-5-TAMARA-11-ddUTP, 5-FAM-Phe-5-R110-11-ddATP, 5-FAM-Phe-5-R110-11-ddUTP, 5-FAM-Phe-5-R110-11-ddGTP, and 5-FAM-Phe-5-R110-11-ddCTP. An energy transfer cassette may be of the formula: ```wherein F1 is a first dye suitable as an acceptor or donor in an energy transfer arrangement;
```F2 is a second dye that is suitable as a donor or acceptor in an energy transfer arrangement with the first dye;
```B is one or more alkyl groups containing from 1 to 30 atoms, wherein said atoms comprise one or more -NH-, -O-, -CH=CH-, -C I C- and phenylenyl groups, and wherein said atoms are selected from the group consisting of C1-4 linear or branched alkyl, hydroxyl, halo, or phenyl;
```M3 comprises hydrogen or a reactive or functional group adapted for attaching the energy transfer dye to a target material; and
```D comprises an atom or group adapted for attaching F2 to the linker group B.
D
wherein
```L
```F
```B is one or more alkyl groups containing from 1 to 30 atoms, wherein said atoms comprise one or more -NH-, -O-, -CH=CH-, -C I C- and phenylenyl groups, and wherein said atoms are selected from the group consisting of C
```M
```D comprises an atom or group adapted for attaching F
Inventors:
Shiv Kumar
Satiam Nampar
Mahesh cot
Satiam Nampar
Mahesh cot
Application Number:
JP2000567958A
Publication Date:
July 30, 2002
Filing Date:
August 30, 1999
Export Citation:
Assignee:
Amersham Pharmacia Biotech Incorporated
International Classes:
C07D407/14; C07K1/13; C07K5/033; C09B11/04; C09B11/28; C09B23/00; C09K9/02; C12N15/09; C12Q1/68; G01N33/58; C12Q1/6818; G01N21/78; G01N33/542; (IPC1-7): G01N33/58; C07K1/13; C07K5/033; C09B11/28; C09K9/02; C12N15/09; C12Q1/68; G01N21/78
Domestic Patent References:
| JPH09104825A | 1997-04-22 | |||
| JPH1088124A | 1998-04-07 | |||
| JPH06230007A | 1994-08-19 |
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)