Title:
酵素の除去方法および該方法を用いるリン脂質の塩基転移または加水分解方法
Document Type and Number:
Japanese Patent JP4650746
Kind Code:
B2
Abstract:
The invention provides a method of removing an enzyme from a liquid enzyme reaction mixture used in a hydrolysis reaction or a base exchange reaction of a phospholipid. The method according to the present invention includes the step of treating the liquid enzyme reaction mixture with a solvent mixture of water and an organic solvent, wherein the solvent mixture includes an inorganic metal salt, to remove the enzyme. According to the present invention, enzymes included in the reaction product can be easily removed without a treatment such as heating, and thus it becomes possible to easily produce various phospholipids that have a reduced risk of inducing an allergy, that retain a high quality and that have excellent storage stability.
Inventors:
Liu Xiao Li
Taniwaki Shigeyuki
Taniwaki Shigeyuki
Application Number:
JP2006511336A
Publication Date:
March 16, 2011
Filing Date:
March 18, 2005
Export Citation:
Assignee:
Nagase Chemtex Co., Ltd.
International Classes:
C12P9/00; C12N9/16
Domestic Patent References:
| JP2002193982A | 2002-07-10 | |||
| JP2003093086A | 2003-04-02 | |||
| JPS63233750A | 1988-09-29 | |||
| JP2004024133A | 2004-01-29 | |||
| JPH07222592A | 1995-08-22 | |||
| JPS62262998A | 1987-11-16 |
Attorney, Agent or Firm:
Takuya Shindo