Title:
高温ポリメラーゼ活性を有する酵素およびその使用方法
Document Type and Number:
Japanese Patent JP2004523211
Kind Code:
A
Abstract:
The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90.degree. C. up to 113.degree. C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.
Inventors:
Karen Walter
Masa Eric J.
Short Jay M
Masa Eric J.
Short Jay M
Application Number:
JP2002525742A
Publication Date:
August 05, 2004
Filing Date:
September 06, 2001
Export Citation:
Assignee:
Divasa Corporation
International Classes:
C12N15/09; C07K16/40; C12M1/00; C12N9/12; C12N15/54; C12P19/34; C12P21/08; C12Q1/48; C12Q1/68; G06F17/30; (IPC1-7): C12N15/09; C07K16/40; C12M1/00; C12N9/12; C12P19/34; C12Q1/48; C12Q1/68; G06F17/30
Attorney, Agent or Firm:
Minoru Nakamura
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda