Title:
二本鎖ＲＮＡ阻害を用いる遺伝子機能の評価
Document Type and Number:
Japanese Patent JP5847776
Kind Code:
B2
Abstract:
A plant cell comprises a DNA sequence from a plant pest which is critical for the plant pest's survival, growth, proliferation or reproduction. The DNA sequence or a fragment thereof is cloned in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). The plant pest may be a plant pest that feeds on the plant. Also provided is a plant comprising the plant cell. Also provided is a method of producing a plant resistant to plant pest infestation, which method comprises identifying a DNA sequence from said pest which is critical for its survival, growth, proliferation, or reproduction. The method also comprises cloning the DNA sequence or a fragment thereof in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). The method also comprises introducing said vector into the plant.
Inventors:
Hert Praetink
Christo Pratteu
Katerin Mortier
Thierry Boguerre
Christo Pratteu
Katerin Mortier
Thierry Boguerre
Application Number:
JP2013186110A
Publication Date:
January 27, 2016
Filing Date:
September 09, 2013
Export Citation:
Assignee:
DEVGEN nv
International Classes:
A01K67/02; C12N15/09; A01N63/00; C12N15/113; A01P5/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/10; C12N15/63; C12N15/82; C12N15/86; C12Q1/02; C12Q1/68; C12Q
Domestic Patent References:
| JP4507083A |
Foreign References:
| WO1998012335A1 | ||||
| WO1998006750A1 | ||||
| WO1997034926A1 |
Other References:
Nature,1998年 2月,Vol.391,p.806-811
Trends in Genetics,1998年 7月 1日,Vol.14, No.7,p.255-258
Nucleic Acids Research,1994年,Vol.22, No.9,p.1762-1763
Trends in Genetics,1998年 7月 1日,Vol.14, No.7,p.255-258
Nucleic Acids Research,1994年,Vol.22, No.9,p.1762-1763
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Kenji Tomita
Fumio Inai
Hiroshi Yamazaki
Kenji Tomita
Fumio Inai