Title:
FILAMENTOUS FUNGAL MUTANT WITH IMPROVED HOMOLOGOUS RECOMBINATION EFFICIENCY
Document Type and Number:
Japanese Patent JP2011152148
Kind Code:
A
Abstract:
To provide a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for non-homologous recombination (NHR).
There is provided a method comprising a step of leading an integration pathway towards homologous recombination (HR) in which the polynucleotide has a region of homology with the pre-determined site. The filamentous fungus mutant originating from a parent cell has an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or an NHR/HR ratio with decreased efficiency as compared to the HR and/or NHR efficiency and/or NHR/HR ratio of the parent cell under the same conditions.
Inventors:
DEKKER PETRUS JACOBUS THEODORUS
BERG VAN DEN MARCO ALEXANDER
BERG VAN DEN MARCO ALEXANDER
Application Number:
JP2011085638A
Publication Date:
August 11, 2011
Filing Date:
April 07, 2011
Export Citation:
Assignee:
DSM IP ASSETS BV
International Classes:
C12N15/09; C07K14/37; C07K14/38; C07K14/385; C12N1/14; C12N1/15; C12N15/80; C12N15/90; C12P21/02; C12P23/00; C12P37/00
Domestic Patent References:
| JP2003526376A | 2003-09-09 |
Foreign References:
| WO2002052026A2 | 2002-07-04 |
Other References:
JPN6010035318; 第２６回日本分子生物学会年会プログラム・講演要旨集、２００３年１１月２５日発行、ｐ． ８３０、３ＰＡ-２１４
JPN6010035322; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， （１９９６）， Ｖｏｌ． ２４， Ｎｏ． ２３， ｐ． ４６３９-４６５０
JPN6010035315; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， （１９９６）， Ｖｏｌ． ２４， Ｎｏ． １１， ｐ． ２０６７-２０７２
JPN6010035322; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， （１９９６）， Ｖｏｌ． ２４， Ｎｏ． ２３， ｐ． ４６３９-４６５０
JPN6010035315; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， （１９９６）， Ｖｏｌ． ２４， Ｎｏ． １１， ｐ． ２０６７-２０７２
Attorney, Agent or Firm:
Yuichi Yamada
Masakazu Noda
Yoshinori Shimizu
Ikeda adult
Kazuhiro Yamaguchi
Kimoto
Masakazu Noda
Yoshinori Shimizu
Ikeda adult
Kazuhiro Yamaguchi
Kimoto