Title:
表面上の液体層中の生物学的活性分子のためのフロースルー剪断分析器
Document Type and Number:
Japanese Patent JP3935317
Kind Code:
B2
Abstract:
The adsorption rate of proteins from solutions on surfaces in the region of interface layers is often so large that a depletion of the protein in the interface layer results. Due to this, the total reaction becomes transport-dependent, sensitively disrupting the determination of the rate constants. In known TIRF-analysis chambers or bio-sensor systems with a liquid interface layer of ~10 mum thickness and mass transport coefficients of 10<-6>-10<-5 >m/s it has up limitation.With the help of a TIRF-flow-through shear analyzer in which a certain volume unit of an immiscible fluid, for example an air bubble, is fed into the buffer flow, an ultra-thin liquid layer arises on the surface with a thickness of 100-200 nm, wherein interface surfaces below 10 nm thickness are technically possible.The new TIRF-flow-through shear analyzer therefore allows the generation of ultra-thin liquid layers while increasing the mass transport coefficients for proteins by 50-100-fold so that the sorption rate constants can be determined without transport limitation.
Inventors:
Herbert Peter Jennissen
Thomas Tsumblink
Thomas Tsumblink
Application Number:
JP2000551244A
Publication Date:
June 20, 2007
Filing Date:
May 25, 1999
Export Citation:
Assignee:
Herbert Peter Jennissen
International Classes:
G01N21/27; G01N21/64; G01N13/00; G01N21/03; G01N21/05; G01N21/43; G01N21/55; G01N1/34
Domestic Patent References:
| JP8145879A | ||||
| JP257947A | ||||
| JP11183372A | ||||
| JP52130061A | ||||
| JP310902B2 | ||||
| JP37270B2 | ||||
| JP4501462A | ||||
| JP1500928A | ||||
| JP7507865A | ||||
| JP2002509248A |
Foreign References:
| WO1997039338A1 | ||||
| WO1997009618A1 | ||||
| EP0378968A2 | ||||
| EP0047130A2 | ||||
| RU2095787A |
Attorney, Agent or Firm:
Hisayoshi Shimizu
Ken Takada
Kurose Yasuhisa
Masaki Kobayashi
Ken Takada
Kurose Yasuhisa
Masaki Kobayashi