Title:
FLUORESCENCE IMAGE ANALYSIS METHOD, FLUORESCENCE IMAGE ANALYSIS APPARATUS, FLUORESCENCE IMAGE ANALYSIS PROGRAM
Document Type and Number:
Japanese Patent JP2023027845
Kind Code:
A
Abstract:
To provide an optical image analysis device or the like that outputs a ratio of positive cells without requiring cell isolation.SOLUTION: A fluorescence image analysis apparatus 10 includes an imaging unit 154 that captures a first image including at least a partial region of a plurality of cells in a sample in which target sites on chromosomes are labeled with a fluorescent dye and a second image including, as an imaging target, fluorescence generated from a fluorescent dye that labels the target site of cells in the first image. A processing unit 11 of the fluorescence image analysis device 10 selects a plurality of test cells, which have specific morphological characteristics and are to be tested, from the plurality of cells based on at least the first image, and extracts bright spots due to fluorescence generated from the fluorescent dye from at least the plurality of test cells based on the second image. The processing unit 11 identifies cells with chromosomal abnormality and/or cells without chromosomal abnormality based on the extracted bright spots, and generates information about a ratio of cells with chromosomal abnormality to the test cells based on the number of cells with chromosomal abnormality and/or the number of cells without chromosomal abnormality.SELECTED DRAWING: Figure 1
Inventors:
INASAWA JOJI
KURODA JUNYA
KINOSHITA MASAKI
KURODA JUNYA
KINOSHITA MASAKI
Application Number:
JP2021133166A
Publication Date:
March 03, 2023
Filing Date:
August 18, 2021
Export Citation:
Assignee:
UNIV TOKYO MEDICAL & DENTAL
KYOTO PREFECTURAL PUBLIC UNIV CORP
SYSMEX CORP
KYOTO PREFECTURAL PUBLIC UNIV CORP
SYSMEX CORP
International Classes:
G01N21/64; C12M1/34; C12Q1/02; C12Q1/68; G01N33/48; G01N33/483; G01N33/53
Attorney, Agent or Firm:
Patent Attorney Corporation YKI International Patent Office