Title:
ヒスチジンデカルボキシラーゼ活性を測定するための蛍光偏光アッセイ
Document Type and Number:
Japanese Patent JP2008520977
Kind Code:
A
Abstract:
The present invention relates to fluorescence polarization assays for determining the HDC modulating activity of a candidate compound including providing a reaction mixture comprising a HDC, histidine, a fluorescently labeled histamine probe, a candidate compound and an anti histamine antibody having selectivity for histamine at least 10 fold greater than histidine: incubating the reaction mixture; and determining whether inhibition of HDC has occurred in the presence of the test compound, wherein an increase in fluorescence signal is an indication that the test compound inhibits the activity of the HDC.
Inventors:
August E Michael
Rajote Daniel
Rajote Daniel
Application Number:
JP2007541490A
Publication Date:
June 19, 2008
Filing Date:
November 15, 2005
Export Citation:
Assignee:
Boehringer Ingelheim Pharmaceuticals, Inc.
International Classes:
G01N33/53; C12Q1/527; G01N21/64; G01N21/78; G01N33/15; G01N33/50; G01N33/542; G01N33/573; C12N9/88
Domestic Patent References:
| JP2001513755A | 2001-09-04 | |||
| JP2000512737A | 2000-09-26 | |||
| JPH09110857A | 1997-04-28 | |||
| JPH1183855A | 1999-03-26 |
Foreign References:
| WO2004068115A2 | 2004-08-12 |
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama