Title:
FUNCTIONAL DOMAIN OF DOCK2 INDISPENSABLE TO WANDERING OF LYMPHOCYTE AND ASSOCIATED MOLECULE
Document Type and Number:
Japanese Patent JP3886983
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a screening method for a substance interfering with the association of DOCK2 and ELMO1, a screening method for a substance interfering with the association of ELMO1 and Tiam1 or a method for searching a treatment medicine for an immune related disease such as allergy, autommunity, GvH, graft rejection or the like.
SOLUTION: It is found out that an Rac activation capacity is markedly lowered in a DOCK2 mutant lacking of the 504 amino acid residue of the N terminal of DOCK2 and actin polymerization can not be brought about, to identify MLMO1 as a molecule bonded to this region. It is found out that DOCK2 is associated with ELMO1 through an SH3 domain. Further, it is found out that ELMO1 is bonded to Tiam1 functioning as an Rac specific GDP/GTP exchange factor (GEF) and it is found out that Rac is activated by recruiting Tiam1 by DOCK2 through ELMO1.
Inventors:
Nobunori Fukui
Takehiko Sasazuki
Takehiko Sasazuki
Application Number:
JP2004128900A
Publication Date:
February 28, 2007
Filing Date:
April 23, 2004
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
G01N33/50; C07K14/47; G01N33/15; G01N33/53; G01N33/566; (IPC1-7): G01N33/50; G01N33/15; G01N33/53; G01N33/566; //C07K14/47
Other References:
NATURE Vol.412(2001) p.826-831
NATURE Vol.375(1995) p.338-340
CELL Vol.107(2001) p.27-41
BIOCHEMICA ET BIOPHYSICAL RESEARCH COMMUNICATIONS,Vol.1452(1999) , p.179-187
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,Vol.296 (AUGUST 2002) ,p.716-720
NATURE Vol.375(1995) p.338-340
CELL Vol.107(2001) p.27-41
BIOCHEMICA ET BIOPHYSICAL RESEARCH COMMUNICATIONS,Vol.1452(1999) , p.179-187
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,Vol.296 (AUGUST 2002) ,p.716-720
Attorney, Agent or Firm:
Masaki Hirota