FUNCTIONAL DOMAIN IN FLAVOBACTERIUM OKEANOKOITES (FOKI) RESTRICTION ENDONUCLEASE

Japanese Patent JP2013081471

A

To provide a DNA segment identifying recognition and cleavage domains of FokI restriction endonuclease, and encoding each of the recognition and cleavage domains of FokI restriction endonuclease.

A 41kDa N terminus fragment constitutes the recognition domain of the FokI restriction endonuclease and the 41kDa C terminus fragment constitutes a nuclease domain of FokI restriction endonuclease. A hybrid restriction enzyme contains a nuclease domain of the FokI restriction endonuclease connected with a recognition domain of another enzyme. Ubx-F_N is one of such hybrid restriction enzymes. The enzyme contains a Ubx homeodomain connected with the FokI cleavage or nuclease domain. The invention also relates to two insertion mutants of a FokI endonuclease.

CHANDRASEGARAN SRINIVASAN

JP2012279894A

May 09, 2013

December 21, 2012

Click for automatic bibliography generation  

UNIV JOHNS HOPKINS

C12N15/09; C12N1/21; C12N9/16; C12N9/22; C12N15/55; C12Q1/68; C12R1/01; C12R1/19

JPN6011042333; Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｎａｔｉｏｎａｌ　Ａｃａｄｅｍｙ　ｏｆ　Ｓｃｉｅｎｃｅｓ　ｏｆ　ｔｈｅ　Ｕｎｉｔｅｄ　Ｓｔａｔｅｓ　ｏｆ　Ａｍｅｒｉｃａ Ｖｏｌ．８９, 1992, ｐ．４２７５-４２７９
JPN6011042331; Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｎａｔｉｏｎａｌ　Ａｃａｄｅｍｙ　ｏｆ　Ｓｃｉｅｎｃｅｓ　ｏｆ　ｔｈｅ　Ｕｎｉｔｅｄ　Ｓｔａｔｅｓ　ｏｆ　Ａｍｅｒｉｃａ Ｖｏｌ．９０，　Ｎｏ．７, 19930401, ｐｐ．２７６４-２７６８
JPN6011042332; ＥＭＢＯ　Ｊｏｕｒｎａｌ Ｖｏｌ．１０，　Ｎｏ．５, 1991, ｐｐ．１１７９-１１８６

Kurata Masatoshi
Yoshihiro Fukuhara
Makoto Nakamura
Nobuhisa Nogawa
Toshio Shirane
Takashi Mine
Kocho Chojiro
Naoki Kono
Katsu Sunagawa
Morisezo Iseki
Tatsushi Sato
Takashi Okada
Mihoko Horiuchi
Takenori Masanori

Previous Patent: BOTTLED BEVERAGE COMPRISING CAP CONTAINING SUPPLEMENT AND BOTTLE FILLED WITH DISPERSION MEDIUM FOR S...

Next Patent: IL-15 BINDING SITE FOR IL15-Rα AND SPECIFIC IL-15 MUTANT HAVING AGONIST/ANTAGONIST ACTIVITY