Title:
ゲノム編集方法
Document Type and Number:
Japanese Patent JP7029741
Kind Code:
B2
Abstract:
A technique for editing genome using a single strand polynucleotide consisting of: (i) (ia) a modified nucleotide sequence having a deletion of an arbitrary endogenous nucleotide sequence from an arbitrary nucleotide sequence in genomic DNA; (ib) a modified nucleotide sequence having an insertion of an arbitrary nucleotide sequence to an arbitrary nucleotide sequence in genomic DNA; or (ic) a modified nucleotide sequence having a substitution of an arbitrary endogenous nucleotide sequence with another nucleotide sequence in an arbitrary nucleotide sequence in genomic DNA; or (ii) a nucleotide sequence having 90% or more identity with the modified nucleotide sequence (except the same nucleotide sequence as the sense strand nucleotide sequence).
Inventors:
Hideaki Maseda
Good Maki
Good Maki
Application Number:
JP2021150104A
Publication Date:
March 04, 2022
Filing Date:
September 15, 2021
Export Citation:
Assignee:
NEXUS PIRAL Co., Ltd.
National Institute of Advanced Industrial Science and Technology
National Institute of Advanced Industrial Science and Technology
International Classes:
C12N5/10; C12N15/09; C12N15/11
Foreign References:
| WO2004015117A2 |
Other References:
LIU, Jia et al.,Sequence conversion by single strand oligonucleotide donors via non-homologous end joining in mammalian cells,J. Biol. Chem.,2010年,Vol. 285,pp. 23198-23207
Hudson, D. F. et al.,Reverse genetics of essential genes in tissue-culture cells: 'dead cells talking',Trends Cell Biol.,2002年,Vol. 12,pp. 281-287
Majumdar, A. et al.,Targeted gene knock in and sequence modulation mediated by a psoralen-linked triplex-forming oligonucleotide,J. Biol. Chem.,2008年,Vol. 283,pp. 11244-11252
McLachlan, J. et al.,Specific targeted gene repair using single-stranded DNA oligonucleotides at an endogenous locus in mammalian cells uses homologous recombination,DNA Repair,2009年,Vol. 8,pp. 1424-1433
Hudson, D. F. et al.,Reverse genetics of essential genes in tissue-culture cells: 'dead cells talking',Trends Cell Biol.,2002年,Vol. 12,pp. 281-287
Majumdar, A. et al.,Targeted gene knock in and sequence modulation mediated by a psoralen-linked triplex-forming oligonucleotide,J. Biol. Chem.,2008年,Vol. 283,pp. 11244-11252
McLachlan, J. et al.,Specific targeted gene repair using single-stranded DNA oligonucleotides at an endogenous locus in mammalian cells uses homologous recombination,DNA Repair,2009年,Vol. 8,pp. 1424-1433
Attorney, Agent or Firm:
Seiji Ohno
Yu Morita
Yu Morita