ニューロンニコチン性アセチルコリン受容体のβ2-サブユニットの調節配列及びこれをコードする配列を含むゲノムDNA断片、及びこれらの断片又は突然変異断片を用いて作製されたトランスジェニック動物

Japanese Patent JP4450874

B2

Several genes encoding subunits of the neuronal nicotinic acetylcholine receptors have been cloned and regulatory elements involved in the transcription of the alpha :2 and alpha :7-subunit genes have been described. Yet, the detailed mechanisms governing the neuron-specific transcription and the spatio-temporal expression pattern of these genes remain largely uninvestigated. The beta 2-subunit is the most widely expressed neuronal nicotinic receptors subunit in the nervous system. We have studied the structural and regulatory properties of the 5' sequence of this gene. A fragment of 1163 bp of upstream sequence is sufficient to drive the cell-specific transcription of a reporter gene in both transient transfection assays and in transgenic mice. Deletion analysis and site-directed mutagenesis of this promoter reveal two negative and one positive element. The positively acting sequence includes one functional E-box. One of the repressor elements is located in the transcribed region and is the NRSE/RE1 sequence already described in promoters of neuronal genes.

JPWO2014084055	Type I diabetes analysis animals, or polymyositis and / or autoimmune myocarditis model animals
JPWO2003046182	Testicular-derived human glioma antigen
WO/2019/145453	ARGINASE SUPPRESSION FOR CANCER TREATMENT

Jean-Pierre Shangju
Marina Pitch Otto
Alan Bethes

JP32609495A

April 14, 2010

December 14, 1995

Click for automatic bibliography generation  

INSTITUT PASTEUR

A01K67/027; C07H21/04; C12N15/09; C07K14/705; C07K14/715; C12N5/10; C12N15/12; C12N15/85; C12Q1/68

Neuron(1988)Vol.1,No.1,p.45-54
FEBS Lett.(1990)Vol.264,No.1,p.48-52
Nucleic Acids Res.(1993)Vol.21,No.9,p.2185-2192
Development(1992)Vol.115,No.1,p.213-219
J Neurochem.(1994)Vol.62,No.1,p.392-395

Hajime Tsukuni
Fusayuki Saito
Yuichi Okazaki

Previous Patent: 内視鏡用穿刺針操作補助器具

Next Patent: 求電子官能基少なくとも１個を含有する化合物上に置換ジフルオロメチル�...