Title:
GERANYLGERANYL DIPHOSPHATE SYNTHASE AND DNA ENCODING THE SAME
Document Type and Number:
Japanese Patent JP3198842
Kind Code:
B2
Abstract:
PURPOSE: To obtain the subject new DNA encoding geranylgeranyl diphosphate (GGDP)synthase derived from Sulfolobus-acidocaldarius (ATCC 3,3909), capable of producing GGDP useful as a biosynthetic intermediate for carotenoid, diterpene, rubber, etc.
CONSTITUTION: This new DNA has an amino acid sequence of the formula, encodes geranylgeranyl diphosphate (GGDP)synthase derived from Sulfolobus- acidocaldarius (ATCC 33,909) and is capable of producing GGDP useful as a biosynthetic intermediate for carotenoid, diterpene, rubber, etc. The DNA is obtained by collecting a chromosome DNA from Sulfolobus-acidocaldarius, screening a gene library prepared by a conventional procedure and treating a plasmid recovered from a positive clone of the screening with a restriction enzyme.
Inventors:
Ooto Toku
Chika Asada
Yuka Higuchi
Yoshie Takeuchi
Tokuzo Nishino
Shinichi Ohnuma
Manabu Suzuki
Chika Asada
Yuka Higuchi
Yoshie Takeuchi
Tokuzo Nishino
Shinichi Ohnuma
Manabu Suzuki
Application Number:
JP31557294A
Publication Date:
August 13, 2001
Filing Date:
November 25, 1994
Export Citation:
Assignee:
TOYOTA JIDOSHA KABUSHIKI KAISHA
International Classes:
C12N15/09; C12N1/21; C12N9/10; C12N15/00; C12N15/54; C12P7/04; C12P9/00; C12R1/01; C12R1/19; (IPC1-7): C12N15/09; C12N1/21; C12N9/10; C12P7/04
Attorney, Agent or Firm:
Takashi Ishida (3 others)