GLUTAMINE-AUXOTROPHIC HUMAN CELLS CAPABLE OF PRODUCING PROTEINS AND CAPABLE OF GROWING IN A GLUTAMINE-FREE MEDIUM

Title:

GLUTAMINE-AUXOTROPHIC HUMAN CELLS CAPABLE OF PRODUCING PROTEINS AND CAPABLE OF GROWING IN A GLUTAMINE-FREE MEDIUM

Document Type and Number:

Japanese Patent JP2007300935

Kind Code:

A

Abstract:

To provide a glutamine-auxotrophic human cell capable of producing proteins and capable of growing in a glutamine-free medium.

Disclosed is a glutamine-auxotrophic human cell transfected with an exogenous DNA sequence capable of altering the expression of an exogenous DNA sequence encoding a protein or an endogenous gene encoding a protein and with an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more than one DNA construct, and the transfected cell is capable of producing the protein and capable of growing in a glutamine-free medium.

Inventors:

BIRCH JOHN
BORASTON ROBERT CHARLES
SHAW MARTYN

Application Number:

JP2007175977A

Publication Date:

November 22, 2007

Filing Date:

July 04, 2007

Export Citation:

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Assignee:

LONZA BIOLOGICS PLC

International Classes:

C12N5/10; C12N15/09; C07K14/505; C12N5/09; C12N5/22; C12N9/00; C12N15/85; C12P21/02; C12Q1/25

Domestic Patent References:

JPH04505104A	1992-09-10
JPH07500969A	1995-02-02
JPS63502955A	1988-11-02
JPH11502719A	1999-03-09

Attorney, Agent or Firm:

Takehiko Suzue
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Toshio Shirane
Sadao Muramatsu
Ryo Hashimoto
Tetsuya Kazama

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