Title:
熱安定ルシフェラーゼと生産方法
Document Type and Number:
Japanese Patent JP5580607
Kind Code:
B2
Abstract:
Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50°C, cDNAs encoding the novel luciferases, and hosts transformed to express the lucifases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
Inventors:
The Keith buoy ウツド
Mary Py Hall
Mary Py Hall
Application Number:
JP2010006999A
Publication Date:
August 27, 2014
Filing Date:
January 15, 2010
Export Citation:
Assignee:
Promega Condominium ration Promega Corporation
International Classes:
C12N15/09; C12N9/02; C12N15/10; C12N15/53; C12Q1/68; C12N1/21; C12Q1/66
Attorney, Agent or Firm:
辻居 Koichi
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Yamasaki Kazuo
Ichikawa Azalea
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Yamasaki Kazuo
Ichikawa Azalea