HEAT TREATMENT OF IMMOBILIZED CELL - MITSUI TOATSU CHEM INC

Title:

HEAT TREATMENT OF IMMOBILIZED CELL

Document Type and Number:

Japanese Patent JP01132394

Kind Code:

A

Abstract:

PURPOSE: To improve initial activity of enzyme and to enable long-term preservation of an immobilized cell without lowering activity of enzyme, by heat-treating the immobilized cell containing a tryptophan synthase under a specific pH condition.

CONSTITUTION: A tryptophan synthase producing L-tryptophan from indole and L-serine, such as tryptophan synthase produced by Escherichia coli MT-10232 is immobilized by a conventional procedure and the prepared immobilized cell is heat-treated at pH 6W9, preferably pH 6.5W8.0 at ≥about 40°C, preferably about 40W75°C.

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Inventors:

Iguchi, Seiya
Ogawa, Shinji
Noguchi, Takeshi
Kaneko, Kiyoshi

Application Number:

JP1987000289267

Publication Date:

May 24, 1989

Filing Date:

November 18, 1987

Export Citation:

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Assignee:

MITSUI TOATSU CHEM INC

International Classes:

C12P13/22; C12N11/00; C12P13/00; C12N11/00; (IPC1-7): C12N11/00; C12P13/22

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