Title:
高親和性TCRタンパク質および方法
Document Type and Number:
Japanese Patent JP4972264
Kind Code:
B2
Abstract:
T cell receptors (TCRs) that have higher affinity for ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody.
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Inventors:
Clans, David M.
Whitlap, Kay. Dane
Horror, Philip Dee.
Whitlap, Kay. Dane
Horror, Philip Dee.
Application Number:
JP2001548658A
Publication Date:
July 11, 2012
Filing Date:
December 06, 2000
Export Citation:
Assignee:
Board of Trustees of the University of Illinois
International Classes:
C12N15/09; G01N33/53; C07K14/725; C07K14/74; C12N5/10; C12Q1/02; G01N33/566; G01N33/58
Foreign References:
| WO1998039482A1 | ||||
| WO1999036569A1 |
Other References:
Immunity, 8(1998) p.413-425
J. Mol. Biol.,256(1996) p.859-869
J. Immunol. Methods, 220(1998) p.179-188
J. Mol. Biol.,256(1996) p.859-869
J. Immunol. Methods, 220(1998) p.179-188
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita