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Title:
ソノポレーションを使用した生物学的細胞の高効率トランスフェクション
Document Type and Number:
Japanese Patent JP7155318
Kind Code:
B2
Abstract:
A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Inventors:
Jennifer M. Hardy
Richard N. Elson
Richard G. Stearns
Babur Hadimioglu
Joseph D. Olekno
Marsha N. Braukamp
Application Number:
JP2021057414A
Publication Date:
October 18, 2022
Filing Date:
March 30, 2021
Export Citation:
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Assignee:
Love Site Incorporated
International Classes:
C12N15/87; C12N13/00
Domestic Patent References:
JP2011525899A
JP2004535764A
Foreign References:
US20010053384
WO2006105251A2
WO2015110955A1
Other References:
MESTAS, J.L., et al.,"DEVELOPMENT OF A CONFOCAL ULTRASOUND DEVICE USING AN INERTIAL CAVITATION CONTROL FOR TRANSFECTION IN-VITRO.",JOURNAL OF PHYSICS: CONFERENCE SERIES,英国,INSTITUTE OF PHYSICS PUBLISHING,2015年12月03日,Vol.656, No.1,012003(pp.1-4),http://dx.doi.org/10.1088/1742-6596/656/1/012003
Attorney, Agent or Firm:
Shusaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto