Title:
ソノポレーションを使用した生物学的細胞の高効率トランスフェクション
Document Type and Number:
Japanese Patent JP7155318
Kind Code:
B2
Abstract:
A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.
Inventors:
Jennifer M. Hardy
Richard N. Elson
Richard G. Stearns
Babur Hadimioglu
Joseph D. Olekno
Marsha N. Braukamp
Richard N. Elson
Richard G. Stearns
Babur Hadimioglu
Joseph D. Olekno
Marsha N. Braukamp
Application Number:
JP2021057414A
Publication Date:
October 18, 2022
Filing Date:
March 30, 2021
Export Citation:
Assignee:
Love Site Incorporated
International Classes:
C12N15/87; C12N13/00
Domestic Patent References:
JP2011525899A | ||||
JP2004535764A |
Foreign References:
US20010053384 | ||||
WO2006105251A2 | ||||
WO2015110955A1 |
Other References:
MESTAS, J.L., et al.,"DEVELOPMENT OF A CONFOCAL ULTRASOUND DEVICE USING AN INERTIAL CAVITATION CONTROL FOR TRANSFECTION IN-VITRO.",JOURNAL OF PHYSICS: CONFERENCE SERIES,英国,INSTITUTE OF PHYSICS PUBLISHING,2015年12月03日,Vol.656, No.1,012003(pp.1-4),http://dx.doi.org/10.1088/1742-6596/656/1/012003
Attorney, Agent or Firm:
Shusaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto