Title:
HIGH-SENSITIVITY MEASUREMENT METHOD FOR STEROID
Document Type and Number:
Japanese Patent JP2008275425
Kind Code:
A
Abstract:
To provide a method for measuring steroid having a hydroxyl group contained in traces in a living body-derived specimen by using a liquid chromatograph mass spectrometer (LC-MS).
The hydroxyl group of the steroid in the living body-derived specimen is reacted with a compound shown by an expression (I) (in the expression, R is indicative of a hydrogen atom, an alkyl group, an alkoxy group, an allyl alkoxy group, a dialkyl amino group, a nitro group, etc., and COX is indicative of carboxylic acid or its reactive functional group) to form an ester derivative. The ester derivative acquired is measured using the LC-MS.
Inventors:
NUMAZAWA MITSUTERU
YAMASHITA YUKIKAZU
YAMASHITA YUKIKAZU
Application Number:
JP2007118964A
Publication Date:
November 13, 2008
Filing Date:
April 27, 2007
Export Citation:
Assignee:
TEIKOKU ZOKI SEIYAKU MEDICAL K
International Classes:
G01N30/88; G01N27/62; G01N30/06; G01N30/72
Other References:
JPN6011057969; Ａｋｉｒａ Ｈｏｎｄａ ｅｔ ａｌ: 'Ｈｉｇｈｌｙ ｓｅｎｓｉｔｉｖｅ ｑｕａｎｔｉｆｉｃａｔｉｏｎ ｏｆ ７alpha-ｈｙｄｒｏｘｙ-４-ｃｈｏｌｅｓｔｅｎ-３-ｏｎｅ ｉｎ ｈｕｍａｎ ｓｅｒｕｍ ｂｙ ＬＣ-ＥＳＩ-ＭＳ／ＭＳ' Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｌｉｐｉｄ Ｒｅｓｅａｒｃｈ ４８, 200702, ４５８-４６４