Title:
HIRUDIN DERIVATIVE, ITS OBTAINING, RECOMBINANT DNA AND RECOMBINANT VECTOR
Document Type and Number:
Japanese Patent JPH04211391
Kind Code:
A
Abstract:
PURPOSE: To provide a method for obtaining a hirudin derivative from an E. coli secretor mutant.
CONSTITUTION: This process for obtaining a hirudin derivative comprises (1) forming a recombinant vector having a gene encoding the hirudin derivative directly on the downstream of a DNA section encoding a bacterial signal peptide, (2) transforming an E. coli secretor mutant with the recombinant vector formed in the process (1), (3) culturing the transformed cell in a culture medium, and (4) obtaining the hirudin derivative from the culture medium.
Inventors:
GERUHARUTO SHIYUMITSUTO
PAURU HAABERUMAN
PAURU HAABERUMAN
Application Number:
JP5671491A
Publication Date:
August 03, 1992
Filing Date:
March 20, 1991
Export Citation:
Assignee:
CONSORTIUM ELEKTROCHEM IND
International Classes:
A61P7/02; C07K14/005; C07K14/815; C12N1/21; C12N9/10; C07K14/195; C12N15/00; C12N15/09; C12N15/12; C12N15/15; C12N15/71; C12N15/72; C12P21/02; C12R1/19; (IPC1-7): C07K15/04; C12N1/21; C12N15/15; C12N15/70; C12P21/02
Domestic Patent References:
| JPH0235077A | 1990-02-05 |
Foreign References:
| EP0352228A2 | 1990-01-24 | |||
| EP0356335A1 | 1990-02-28 |
Attorney, Agent or Firm:
Toshio Yano (2 outside)
Previous Patent: JPS4211390
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