Title:
少なくとも2つの核酸配列間の比率を定量化する方法
Document Type and Number:
Japanese Patent JP2006505268
Kind Code:
A
Abstract:
The invention provides a method for quantifying an initial ratio of the amounts of at least two nucleic acids of interest in a sample by means of a multiplex nucleic acid amplification reaction, comprising:- amplifying said nucleic acids of interest in said amplification reaction; - measuring the amount of at least two nucleic acids of interest at at least two different time points in said reaction; - determining from at least two of said measurements the amplification rate of said at least two nucleic acids of interest; - comparing said rates with a reference; and - determining from said comparison said initial ratio of the amounts of said at least two nucleic acids of interest in said sample. Preferably, at least one variable factor in said nucleic acid amplification reaction is adjusted in order to allow detectable levels of all nucleic acids of interest to be reached before an amplification and/or detection limit of one or more of the nucleic acids of interest is reached. With a method of the invention, small differences between ratios can be determined. Moreover, direct measurement of the initial nucleic acid ratio can be determined. A method of the invention is for instance suitable for determining functioning of a cellular organism, for determining the staging of a disease, and for determining therapeutic activity and/or possible side-effects of a medicament or candidate compound.
Inventors:
Timmermanth, Evelyn Katerina Anna Krasina
Bob Hemen, Bob
Bob Hemen, Bob
Application Number:
JP2004549721A
Publication Date:
February 16, 2006
Filing Date:
November 07, 2003
Export Citation:
Assignee:
PRIMAGEN HOLDING B.V.
International Classes:
C12Q1/68; A61K31/09; A61K31/16; A61K31/185; A61K31/198; A61K31/337; A61K31/352; A61K31/454; A61K31/506; A61K31/513; A61K31/52; A61K31/522; A61K31/5377; A61K31/675; A61K31/7068; A61K31/7072; A61K31/7076; A61K36/18; A61K38/00; A61K38/21; A61P31/18; A61P35/00; A61P37/04; C12N15/09; C12Q1/02
Attorney, Agent or Firm:
Keiichiro Saikyo
Takeshi Sugiyama
Minetarou Hirose
Takeshi Sugiyama
Minetarou Hirose