Title:
染色体を消失させた植物細胞の作出方法
Document Type and Number:
Japanese Patent JP4912890
Kind Code:
B2
Abstract:
The present invention provides a simple aneuploid production process, applicable to all species, without producing unexpected damage to chromosomes other than a chromosome which is to be disappeared. A vector comprising two site-specific recognition sequences oriented in the opposite direction, or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence, and a recombinase gene is introduced into a plant cell, or a vector comprising two site-specific recognition sequences oriented in the opposite direction, or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence is introduced into a plant cell; a recombinase is allowed to act transiently in the cell during at least growth of the cell; and the cell is cultured and grown; and a cell in which a predetermined chromosome is disappeared is selected.
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Inventors:
Kazuya Nanto
Masashi Ebinuma
Masashi Ebinuma
Application Number:
JP2006546794A
Publication Date:
April 11, 2012
Filing Date:
December 08, 2005
Export Citation:
Assignee:
NIPPON PAPER INDUSTRIES CO.,LTD.
International Classes:
C12N5/10; A01H5/00; C12N15/09
Other References:
J.Ferment.Bioeng.,1994,77(2),p.125-30
Proc.Natl.Acad.Sci.USA,1994,91(5),p.1706-10
Nucleic.Acids Res.,1995,23(3),p.485-90
Nat.Biotechnol.,2004-Apr,22(4),p.455-8
Proc.Natl.Acad.Sci.USA,1994,91(5),p.1706-10
Nucleic.Acids Res.,1995,23(3),p.485-90
Nat.Biotechnol.,2004-Apr,22(4),p.455-8
Attorney, Agent or Firm:
Shohei Oguri
Hironori Honda
Toshimitsu Ichikawa
Hironori Honda
Toshimitsu Ichikawa