To provide a primer and probe for PCR which can detect murine carinii in high sensitivity without false detection or detection omission: and to provide a method for detecting murine Pneumocystis carinii which can detect murine carinii in high sensitivity without false detection or detection omission.
Disclosed is a method for detecting murine Pneumocystis carinii including a process of performing PCR using DNA extracted from murine lung as a template and using a set of a primer comprising DNA consisting of 40 bases at a maximum which is included in the 3' end of base sequence comprising a specific sequence and another different primer comprising DNA consisting of 40 bases at a maximum which is included in the 3' end of base sequence comprising a specific sequence in the presence of Taqman probe providing DNA containing 50 bases at a maximum including a base sequence of base number 25 to 50 in a base sequence comprising a specific sequence; and a process of detecting DNA amplification by detecting fluorescent light.
COPYRIGHT: (C)2010,JPO&INPIT
Aimi Yanagi
Tachibana Ari
加藤郁之進 監,DNAクローニング1-基本技術-(第2版),宝酒造株式会社,1997年 4月21日,第2版,p.87-88
Pneumocystis carinii f. sp. muris dihydrofolate reductase gene, partial cds. [online]. 31-OCT-2001. NCBI Entrez Nucleotide, ACCESSION AF175561, [Retrieved on 2012-12-3]. Retrieved from the internet:
The Journal of infectious diseases. 2004, Vol.189, No.8, p.1540-1544
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