Title:
細胞の形態学的パラメーターの変化を決定する方法
Document Type and Number:
Japanese Patent JP4498919
Kind Code:
B2
Abstract:
Disclosed is method for measuring a change in a cellular morphological parameter, for example, the position and/or the shape of a cell. The method comprises labelling a population of cells with a marker substance which is in a non-detectable state, but which may be caused to become detectable by exposure to light of a suitable first wavelength. By exposing a sub-population of labelled cells to light of an appropriate first wavelength in a predetermined pattern, the sub-population of cells may be defined in which the area covered by the sub-population conforms to the pattern of illumination used. Subsequent examination of at least a portion of the population of cells using illuminating light of a second wavelength selected to be suitable for detection of the marker, will reveal patterns of distribution of the marker substance indicative of a change in said cellular morphological parameter. Also described and claimed is a method for screening for a test agent whose effect on the cellular morphological parameter is to be determined.
More Like This:
Inventors:
Thomas, Nicholas
Application Number:
JP2004505685A
Publication Date:
July 07, 2010
Filing Date:
May 07, 2003
Export Citation:
Assignee:
GE Healthcare UK Limited
International Classes:
C12Q1/02; G01N33/48; G01N33/483; G01N33/50; G01N33/58
Foreign References:
| WO2001011340A1 |
Other References:
J Neurosci Methods,(2000), vol.104, no.1, p.87-91,p.87-91
Neuron,(1995), vol.15, no.4,p.755-760
Proc Natl Acad Sci USA,(1994), vol.91, no.14,p.6629-6633
Neuron,(1995), vol.15, no.4,p.755-760
Proc Natl Acad Sci USA,(1994), vol.91, no.14,p.6629-6633
Attorney, Agent or Firm:
Arakawa Satoshi
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa