Title:
微生物の判別方法
Document Type and Number:
Japanese Patent JP6830593
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method of distinguishing microorganisms from scattered light patterns of microbial colonies using a lensless imaging system.SOLUTION: A method distinguishes microbes using a lensless imaging system that uses a photo sensor and comprises: a step of culturing a sample including the microbes in a culture vessel placed above the photo sensor and imaging colonies that formed during the incubation period over time; a step of calculating, from characteristics extracted from a plurality of colony-forming images, at least three parameters including colony maximum growth rate μand relative average luminance, or at least two parameters including at least deviation G of a histogram and donut properties D, or donut properties D and image entropy H; and a step of identifying the microbe by referencing and making comparisons on a database created beforehand with parameters calculated for each known microbe.SELECTED DRAWING: Figure 1
Inventors:
Tsuyoshi Tanaka
Tadashi Matsunaga
Yoshino Tomoko
Maeda Yoshimasa
Manabu Harada
Yasushi Hayashi
Tadashi Matsunaga
Yoshino Tomoko
Maeda Yoshimasa
Manabu Harada
Yasushi Hayashi
Application Number:
JP2016172041A
Publication Date:
February 17, 2021
Filing Date:
September 02, 2016
Export Citation:
Assignee:
National University Corporation Tokyo University of Agriculture and Technology
Malcolm Co., Ltd.
Malcolm Co., Ltd.
International Classes:
C12Q1/04; C12M1/34; G01N21/17; G01N33/48
Domestic Patent References:
| JP2003116593A | ||||
| JP2015154729A |
Other References:
Biosens. Bioelectron.,2009年,Vol. 24,pp.1685-1692
Attorney, Agent or Firm:
Alga Patent Office, a patent business corporation
Toshio Takano
Toshio Nakajima
Masaki Murata
Hiroto Yamamoto
Toshio Takano
Toshio Nakajima
Masaki Murata
Hiroto Yamamoto