Title:
L-バリンを微生物により製造する方法
Document Type and Number:
Japanese Patent JP4638048
Kind Code:
B2
Abstract:
The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.
Inventors:
Egering Rotal
Zam Hermann
Zam Hermann
Application Number:
JP2000601187A
Publication Date:
February 23, 2011
Filing Date:
February 21, 2000
Export Citation:
Assignee:
Forschungszentrum Jürich Gesellschaft Mitt Beschlenktel Haftung
International Classes:
C12P13/08; C12N1/21; C12N9/00; C12N9/10; C12N9/88; C12N15/09; C12P13/06; C12R1/15
Domestic Patent References:
| JP8089249A | ||||
| JP5344893A | ||||
| JP2000166580A | ||||
| JP6277067A | ||||
| JP2042988A |
Foreign References:
| WO1996006926A1 |
Other References:
Nucl. Acids Res.,1987年,Vol.15, No.5,P.2137-2155
GENE,1993年,Vol.137, No.2,P.179-185
Appl. Environ. Microbiol.,1999年 5月,Vol.65, No.5,P.1973-1979
J. Bacteriol.,1993年,Vol.175, No.17,P.5595-5603
FEMS Microbiol. Lett.,1996年,Vol.143,P.247-252
J. Bacteriol.,1998年,Vol.180, No.16,P.4056-4067
GENE,1993年,Vol.137, No.2,P.179-185
Appl. Environ. Microbiol.,1999年 5月,Vol.65, No.5,P.1973-1979
J. Bacteriol.,1993年,Vol.175, No.17,P.5595-5603
FEMS Microbiol. Lett.,1996年,Vol.143,P.247-252
J. Bacteriol.,1998年,Vol.180, No.16,P.4056-4067
Attorney, Agent or Firm:
Mitsufumi Esaki
Katsunori Uenishi
Ichiro Torayama
Tsuneo Mihara
Blacksmith
Katsunori Uenishi
Ichiro Torayama
Tsuneo Mihara
Blacksmith