Title:
核酸を分離する方法
Document Type and Number:
Japanese Patent JP4531888
Kind Code:
B2
Abstract:
The present invention relates to methods for separating nucleic acids from other cellular debris, especially substances that carry a net positive charge at low pH, by electrophoresis under acid conditions. In the purification method of the present invention, nucleic acids are separated from proteins found in the same biological sample by applying the sample to an electrophesis gel and subjecting the sample to electrophoresis under acid conditions to separate the nucleic acids from the proteins. The optimum pH may differ for different sample types but can be readily determined by those skilled in the art. Preferably, the separation is performed at a pH of about 2 to about 4. More preferably, electrophoresis is carried out at a pH of 2.5
Inventors:
Carol Ann Creeder
John Wesley Bacchus
John Wesley Bacchus
Application Number:
JP25207299A
Publication Date:
August 25, 2010
Filing Date:
August 04, 1999
Export Citation:
Assignee:
Ortho-Clinical Diagnostics, Incorporated
International Classes:
C12N15/09; G01N27/447; B01D57/02; B03C5/00; C07H1/08; C07H21/00; C12N15/10; C12Q1/68; G01N33/50
Domestic Patent References:
| JP3502043A |
Foreign References:
| WO1998030571A1 |
Other References:
Jannette Carey,Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study,Proceedings of the National Academy of Sciences of the United States of America,1988年,vol. 85, No. 4,p. 975-979
Attorney, Agent or Firm:
Takashi Ishida
Jun Tsuruta
Fukumoto product
Masaya Nishiyama
Higuchi Souji
Jun Tsuruta
Fukumoto product
Masaya Nishiyama
Higuchi Souji