Title:
HUMAN CHOLECYSTOKININ B RECEPTOR
Document Type and Number:
Japanese Patent JP3461852
Kind Code:
B2
Abstract:
PURPOSE: To provide a novel polypeptide useful as a reagent for detecting a hormone, cholecystokinin, existing in the gastrointestinal cells and cerebral cells of a mammalian or useful for screening the agonist, antagonist of the cholecystokinin B receptor.
CONSTITUTION: A polypeptide containing an amino acid sequence ranging from the first Met to the 447th Gly among the amino acid sequence of human cholecystokinin (hereinafter referred to as CCK) B receptor originated from a human brain cell. The polypeptide is obtained by producing a CDNA library from a RNA originated from the human brain cell by a known method, screening the library with the CDNA of the receptor of gastrin similar to the CCK as a probe, introducing the obtained CDNA coding the human CCK-B receptor into a vector, transforming a host cell with the expression vector, and subsequently culturing the transformant.
Inventors:
Kazuo Chihara
Tsutomu Chiba
Toshimitsu Matsui
Mitsuhiro Ito
Taizo Taniguchi
Hirohisa Nakata
Tsutomu Chiba
Toshimitsu Matsui
Mitsuhiro Ito
Taizo Taniguchi
Hirohisa Nakata
Application Number:
JP25702292A
Publication Date:
October 27, 2003
Filing Date:
September 25, 1992
Export Citation:
Assignee:
Shionogi Pharmaceutical Co., Ltd.
International Classes:
C07K14/705; C07K14/00; C07K14/37; C07K14/41; C07K14/595; C12N5/00; C12N5/10; C12N15/09; C12N15/12; C12N15/85; C12P21/02; C12R1/91; (IPC1-7): C07K14/595; C12N5/00; C12N15/09
Attorney, Agent or Firm:
Shusaku Yamamoto