PURPOSE: To provide the gene coding a human parvovirus (HPV) antigen protein, enabling to massively produce the antigen protein, capable of being utilized for the high sensitive measurement of the HPV and the HPV-resistant antibody in a specimen, and useful for the diagnoses of HPV-originated diseases, etc.
CONSTITUTION: The serum of a patient infected with infectious erythema is diluted with a phosphate-buffered physiological saline (pH: 7.2), mixed with proteinase K and sodium dodecyl sulfate, subjected to their reaction at 37°C for 1hr, mixed with phenol, chloroform and isoamyl alcohol, and subsequently centrifuged to recover the supernatant liquid. The supernatant liquid is added to ethanol and subsequently allowed to stand at -20°C to obtain the precipitates of human parvovirus DNA. The DNA is multiplied by a PCR method, and then treated with a restriction enzyme to obtain the objective human parvovirus gene enabling the mass production of the human parvovirus antigen protein and enabling the measurement the human parvovirus or the human parvovirus- resistant antibody in a specimen in high sensitivity.
MATSUNAGA YASUKO
TAKEDA NAOKAZU
MATSUURA ZENJI
OGAWA HIROYUKI
SHIMIZU HIDEHARU
KAMATA KUNIO
KUROSAWA DAISUKE
DENKA SEIKEN KK