To provide a hybridization device capable of hybridizing a tissue- derived biomaterial efficiently as desired with a specific binding material included in a spot region formed on a biochemical analytical unit, and generating biochemical analytical data having excellent quantitativeness.

This hybridization device is characterized by being equipped with a hybridization vessel 9 for storing a hybridization solution 8 containing the tissue-derived biomaterial labeled with a labeling material, electrodes 13a, 13b, a holding part 11 for holding the biochemical analytical unit 1 equipped with a substrate 2 where a plurality of adsorptive regions 4 including the specific binding material are formed mutually separately, in the hybridization solution so that its surface is in contact with the electrode 13a, and a direct current power source 12 for applying a voltage to the electrodes 13a, 13b so as to generate an electric field crossing the biochemical analytical unit held by the holding part 11.