Title:
IL-12活性を増加させるIL-12p40小単位体突然変異遺伝子及びこれをDNAワクチン免疫増強剤として利用する用途
Document Type and Number:
Japanese Patent JP4180826
Kind Code:
B2
Abstract:
The present invention relates to the IL-12p40 subunit mutant gene which can produce IL-12 (interleukin 12) of human and mouse origin with high activity, the expression vector including above mutant gene and the use of them to DNA vaccine adjuvant. Particularly, it relates to IL-12p40 mutant gene which inhibits the secretion of IL-12p40 but normally secretes active IL- 12p70 by making mutation at Asn-222 (human) or Asn- 220 (mouse) amino acid of IL-12p40, which acts as a competitive inhibitor of active form of IL-12, IL-12p70. Therefore, the IL-12p40 mutant gene of the present invention can be useful for DNA vaccination and gene therapy against various diseases, for example, AIDS, hepatitis C or hepatitis B, cancer, influenza, tuberculosis and malaria, which essentially require cellular immune responses for their therapy.
Inventors:
Song, Young, Chu
Lee, Son, Hee
Ha, Sang, Jun
Son, Mann, Ki
Jun, Jun
Lee, Son, Hee
Ha, Sang, Jun
Son, Mann, Ki
Jun, Jun
Application Number:
JP2001567286A
Publication Date:
November 12, 2008
Filing Date:
March 15, 2001
Export Citation:
Assignee:
Genexin Company Limited
International Classes:
C12N15/09; A61K39/00; A61K39/002; A61K39/04; A61K39/145; A61K39/21; A61K39/29; A61K39/39; A61K48/00; A61P31/14; A61P31/16; A61P31/18; A61P31/20; A61P33/06; A61P35/00; A61P37/04; A61P39/04; C07K14/18; C07K14/54; C12N15/24
Foreign References:
| EP0818534A1 |
Other References:
J. Immunol.,1999年,Vol.163,p.2902-2908
J. Immunol.,2000年,Vol.164,p.4752-4761
Nat. Biotechnol.,2002年,Vol.20,p.381-386
J. Immunol.,2000年,Vol.164,p.4752-4761
Nat. Biotechnol.,2002年,Vol.20,p.381-386
Attorney, Agent or Firm:
Kenzo Hara International Patent Office