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Title:
【発明の名称】熱、酸及び/またはアルカリに高い安定性を有する細菌由来突然変異体α―アミラーゼ
Document Type and Number:
Japanese Patent JP3086249
Kind Code:
B2
Abstract:
Thermostable and acid stable alpha -amylases are provided as expression products of genetically engineered alpha -amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant alpha -amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.

Inventors:
Crows Wilhelms Johannes
La Roche Eve
Volebrecht Adrinus Wilhelms Hermanus
Stan Saint Patrick
Roverey marc
Application Number:
JP50995990A
Publication Date:
September 11, 2000
Filing Date:
June 27, 1990
Export Citation:
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Assignee:
Genenco International Inc.
Plant Genetic Systems Namrose Fennault Shap
International Classes:
C12N1/21; C12N9/26; C12N9/28; C12N15/09; C12N15/56; C12N15/70; C12N15/75; C12S3/12; C12P19/14; D06L1/14; D06M16/00; C12R1/07; C12R1/10; C12R1/125; C12R1/19; C12N; (IPC1-7): C12N9/28; D06L1/14; C12P19/14; C12N15/09; C12N1/21
Other References:
【文献】Protein Eng.1(3)p.241(1987)
Attorney, Agent or Firm:
Minoru Nakamura (6 outside)