Title:
【発明の名称】誘電泳動によるバイオエレクトロニック・チップ上の生体粒子のチャネルレス分離
Document Type and Number:
Japanese Patent JP2002502047
Kind Code:
A
Abstract:
The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge ( 10 ) including a microfabricated silicon chip ( 12 ) on a printed circuit board ( 14 ) and a flow cell ( 16 ) mounted to the chip ( 12 ) to form a flow chamber. The cartridge ( 10 ) also includes output pins ( 22 ) for electronically connecting the cartridge ( 10 ) to an electronic controller. The chip ( 12 ) % includes a plurality of circular microelectrodes ( 24 ) which are preferably coated with a protective permeation layer. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.
Inventors:
Jin Chen
Edward El Sheldon The Third
Ray Woo
James P. O'Connell
Edward El Sheldon The Third
Ray Woo
James P. O'Connell
Application Number:
JP2000529896A
Publication Date:
January 22, 2002
Filing Date:
January 26, 1999
Export Citation:
Assignee:
NANOGEN,INC.
International Classes:
B01D57/02; B01J19/00; B01J19/08; B01L3/00; B03C5/00; B03C5/02; C12N13/00; C12N15/09; C12Q1/68; G01N27/447; G01N33/53; G01N37/00; (IPC1-7): G01N27/447; B01D57/02; B01J19/00; B01J19/08; B01L3/00; B03C5/00; C12N15/09; C12Q1/68; G01N33/53; G01N37/00
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)