Title:
【発明の名称】スキャフォルド・タンパク質のランダム・ペプチドライブラリィとの融合
Document Type and Number:
Japanese Patent JP2002526108
Kind Code:
A
Abstract:
The invention relates to the use of fluorescent proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the random peptides and random peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening random peptide libraries. N-terminal, C-terminal, dual N- and C-terminal and one or more internal fusions are all contemplated. Novel fusions utilizing self-binding peptides to create a conformationally stabilized fusion domain are also contemplated.
Inventors:
David Anderson
Jacob Maria Bogenberger
Bo Robert Peel
Jacob Maria Bogenberger
Bo Robert Peel
Application Number:
JP2000574670A
Publication Date:
August 20, 2002
Filing Date:
October 08, 1999
Export Citation:
Assignee:
Rigel Pharmaceuticals Incorporated
International Classes:
C12N15/00; C07K14/435; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N7/00; C12N7/01; C12N9/02; C12N9/86; C12N15/10; C12N15/12; C12N15/62; C12Q1/68; C12Q1/6897; (IPC1-7): C12N15/00; C07K14/435; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N7/00; C12N9/02; C12N9/86; C12Q1/68
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)