Title:
【発明の名称】オキソ化合物、特にアセトフエノンの酵素還元法及びこれに適する酵素
Document Type and Number:
Japanese Patent JP2767164
Kind Code:
B2
Abstract:
A phenylethanol dehydrogenase capable of catalyzing the reduction of acetophenone to R(+)-phenylethanol in the presence of NADPH was isolated from Lactobacilli such as Lactobacillus kefir. The dehydrogenase is also capable of catalyzing the reduction of aromatic, alicyclic and aliphatic ketones selected from the group consisting of p-bromoacetophenone, methylcyclohexanone, acetone, methyl hexyl ketone, 4-phenyl-2-butanone, 1-phenyl-1,2-propanedione, ethyl pentyl ketone, pinacolone, propiophenone and p-chloroacetophenone. The dehydrogenase is rapidly inactivated by EDTA, but conventional inhibitors, chelators and SH-protecting reagents have only a slight effect on activity. The enzyme has a KM of 6x10-4 M for acetophenone. The dehydrogenase is capable of catalyzing the enzymatic reduction of carbonyl compounds to form optically active hydroxy compounds in the presence of NADPH. In such reactions, NADPH can be simultaneously regenerated in the presence of glucose 6-P and glucose-6-P dehydrogenase or isopropanol.
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Inventors:
UERUNERU FUMUMERU
Application Number:
JP10165891A
Publication Date:
June 18, 1998
Filing Date:
May 07, 1991
Export Citation:
Assignee:
FUORUSHUNGUSUTSUENTORUMU YUURITSUHI GMBH
International Classes:
C12N9/04; C12P7/02; C12P7/22; C12P7/26; C12P41/00; C12R1/225; (IPC1-7): C12N9/04; C12P7/22
Attorney, Agent or Firm:
Mitsufumi Esaki (2 others)